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The following is the text of Hokkaido University, Kansai University, and Tokyo Institute of Technology joint press release headlined "DNA origami to scale-up molecular motors" and announced on May 31, 2019.
Joint press release by Hokkaido University, Kansai University, and Tokyo Institute of Technology
DNA origami to scale-up molecular motors
Researchers have successfully used DNA origami to make smooth-muscle-like contractions in large networks of molecular motor systems, a discovery which could be applied in molecular robotics.
The microtubule network contracting in the presence of DNA origami, kinesins, and ATP. (Matsuda K. et al., Nano Letters, April 30, 2019)
“We successfully demonstrated programmed self-assembly of a biomolecular motor system,” write the researchers from Japan and Germany who conducted the study.
The biomolecular motor system, consisting of fibrous microtubules and motor protein kinesins, plays an essential role in cellular transportation systems. Scientists believe they can utilize the motors in molecular robotics but it remains difficult to assemble a larger system from the tiny molecules.
In the current study published in Nano Letters, the research team including Akira Kakugo of Hokkaido University, Akinori Kuzuya of Kansai University, and Akihiko Konagaya of Tokyo Institute of Technology developed a system combining DNA origami and microtubules. The DNA origami were formed from six DNA helices bundled together. Mixing the two components caused the microtubules to self-assemble around the DNA origami forming star-shaped structures. This self-assembly was made possible by the binding of complementary DNA strands attached to each component.
The team then designed a “kinesin linker” which is made of four kinesin motor proteins radiating from a central core protein. These kinesin linkers joined the microtubules together, causing multiple star-like assemblies to connect, forming a much larger hierarchical network.
When adenosine triphosphate (ATP), a molecule which stores and carries energy, was added to the system, the kinesin linkers moved, causing the microtubular network to dynamically contract within a matter of minutes. This resembled the contraction of smooth muscles according to the researchers.
Mixing DNA-modified microtubules, DNA origami and kinesin linkers leads to star-like formations of microtubules that are connected by kinesin linkers. This network contracted dynamically when ATP energy was added. (Matsuda K. et al., Nano Letters, April 30, 2019)
This dynamic contraction only happened when the DNA origami were present, suggesting the importance of the hierarchical assembly within the microtubular network. “Further studies could lead to the use of DNA for controlled, programmable self-assembly and contraction of biomolecular motors. Such motors could find applications in molecular robotics and the development of microvalves for microfluidic devices,” says Akira Kakugo.
Akira Kakugo of Hokkaido University, Akihiko Konagaya of Tokyo Institute of Technology, and Akinori Kuzuya of Kansai University from the research team.
Original article: Matsuda K. et al., Artificial Smooth Muscle Model Composed of Hierarchically Ordered Microtubule Asters Mediated by DNA Origami Nanostructures. Nano Letters, April 30, 2019. DOI: 10.1021/acs.nanolett.9b01201
Funding information: This study was supported by Future AI and Robot Technology Research and Development Project from New Energy and Industrial Technology Development Organization (NEDO), Japan, and Grant-in-Aid for Scientific Research on Innovative Areas “Molecular Engine” (18H05423 and 19H05407), Grant-in-Aid for Challenging Research (Pioneering) (17K19211), Grant-in-Aid for Challenging Exploratory Research (15K12135), and Private University Research Branding Project (2016-2021) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.
Associate Professor Akira Kakugo
Faculty of Science,
Professor Akinori Kuzuya
Department of Chemistry and Materials Engineering,
Department of Computational Intelligence and Systems Science,
Tokyo Institute of Technology
Naoki Namba (Media Officer)
Institute for International Collaboration,
2019/05/31 09:00 UP